Y. Feng1, S. Okuda2, H.Y. Tan1, N. Wang1, S.W. Tsao3, M. Takahashi2,
1 The University of Hong Kong, School of Chinese Medicine, Hong Kong, China
2 Heimat Co,. Ltd, Heimat Building, 1 -2 1 -3 Nihonbashi, Chuo-Ku, Tokyo, Japan;
3 Department of Anatomy, Center of Cancer research, LKS Faculty of Medicine, The University
of Hong Kong
Introduction. various natural products from saw palmetto extract were sold in the world. Saw palmetto extract is an extract of the fruit of Serenoa repens (Bartram) J.K.Small, an edible plant in the southeastern United States. It has been used in traditional, complementary and alternative medicine as a tonic, or an expectorant and antiseptic to treat various ill conditions, especial urinary and reproductive system problems and cancer supportive care. However, there are discrepancy in some studies. The different results may come from different products. Saw palmetto extract is rich in fatty acids and phytosterols. As functional food, NYG, a new natural product isolated from red pigment of Saw palmetto has received positive response in late stage patients with various cancers in Japan, so it is necessary to investigate the effect and mechanism of NYG by scientific research. Objective: To explore the anti-tumor potential of red pigment fraction from Saw Palmetto Extract (NYG) on human hepatocellular carcinoma (HCC).
Material and Method. three products named NYG1, NYG4 and NYG7 were used to evaluate the tumor inhibitory effect of NYGs by in vitro and in vivo HCC models. Xenograft model of HCC, cell migration and blood vessel formation models were used to evaluate the inhibitory effect of NYGs in tumor growth and invasion. The mRNA and protein expression related to HCC after NYGs treatment were analyzed by PCR and western blot.
Result and discussion. Significant tumor inhibition of NYGs in dose-dependent manner in vitro and in vivo HCC models was observed, while NYGSs has no inhibitory effect on the cell viability by MTT assy. NYGs also showed minimal effect on normal hepatic cells, which may suggest the NYGSs safety on patients. These results suggested that the inhibitory effect of NYGSs in tumor growth and invasion may be caused by anti-angiogenesis effect of NYGSs.
Conclusion.NYGs has no cytotoxic effect on HCC cell lines, but it can inhibit cancer growth in in vivo model and cancer blood formation suggesting that NYGs may be used for alternative cancer treatment by inhibiting neovascularization. The study was supported by grants from the University of Hong Kong (Project code: 104002889), Wong’s Donation (Project code: 207040314) and Gaia Family Trust (project code: 200007008).